Abstract
Objective To investigate the change of prostaglandin D2 (DP) receptor on dendritic cells (DC) in exacerbation and remission phase patients with bronchial asthma(asthma) and the ability of receptor to control Th2 polarization. Methods Randomized case control clinical trial was designed. Thirty patients were randomly divided into two groups:exacerbation phase group 32 cases (6.1 ±3.5) years,and remissio nphase group 30 cases (5.8 ± 2.6) years, normal control 34 cases (7. 2± 3.8) years. Radiological binding assay was used to study the DP1/CRTH2 receptors in monocyte-derived DC in peripheral blood. The plasma levels of IL(interleukin) -4,IL-5,and INF-γ were detected by ELISA method. Results The total combining capacity and CRTH2 on DC in peri-pheral blood in exacerbation phase and remission phase group were significantly higher than those in normal controls,total combining capacity: (532.42±123.87) fmol/106cells,(491.781±10. 64) fmol/106 cells,(274.61±87.16) fmol/106 cells. CRTH2:(355.664±99. 19) fmol/106cells,(316. 834±81.42) fmol/106 ce113,(123. 744±49. 27) fmol/106 cells (P <0.01). In three groups,there were no significant differences in DP1. In exacerbation phase and remission phase group, the serum levels of Th2 cytokines IL-4, IL-5 were significantly more increased than in control group, IL-4: (12. 76 4± 3. 54) ng/L,(11. 445±2.65) rig/L, (7.495± 1.45) ng/L. IL-5 : (36.28d±10. 21) ng/L, (32. 11 5±11.56) ng/L, (18. 48 ±5.65) ng/L(P <0. 01). Thl cytokine IFN-γ was significantly decreased in the above three groups, (7. 47 ±2.49) ng/L,(7.565±1.41) ng/L, (13.13 4±1. 44) ng/L(P <0.01). Conclusions There are significantly higher total combining capacity DP and CRTH2 on monocyte-derived DC in exacerbation and remission phase patients with asthma,and the serum levels of Th2 cytokine IL-4,IL-5 are significantly increased. Key words: Bronchial asthma; Dendritic cells; Chemokine receptor-homologs molecule expressed on TH2 cells
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