Change of migration and CD147 expression of human endothelial progenitor cells following traumatic brain injury

Abstract

Objective This study examined the migration and expression of CD147 of human endothelial progenitor cells (EPCs) in severe traumatic brain injury (TBI) patients and their association with clinical outcome.Methods Blood samples were obtained from enrolled 96 severe TBI subjects [Glasgow coma score [GCS] ≤8] and healthy donors.After EPCs were cultured,we studied the temporal profile of the migration ability and expression of CD147 over 6 days.Results Comparison of the migration ability and expression of CD147 in TBI patients versus controls showed significant elevation of the migration ability at 6 h and over the 4 day study period and significant elevation of CD147 at 6 h and over the 3 day (P ＜ 0.05).The migration ability and expression of CD147 in patients with GCS 6-8 at first 12 h and 24 h were significantly higher than those with GCS 3-5 (P ＜ 0.05).The migration ability and expression of CD147 appeared to distinguish severe TBI survivors vs.non-survivors within the study,with survivors having significantly higher migration ability and expression of CD147 (P ＜ 0.05).Furthermore,better migration ability and higher expression of CD147 appeared in good outcome groups than in poor outcome groups (P ＜ 0.05).Conclusion The increased migration ability of EPCs was correlated with increased CD147 after TBI.The migration ability and expression of CD147 in EPCs appear to have potential clinical utility in diagnosing TBI. Key words: Traumatic brain injury; Endothelial progenitor cells; Migration ability; CD147