Abstract

IAA oxidase in Begonia evansiana leaves was partially purified by DEAE cellulose chromatography. The enzyme was identified as peroxidase from its elution pattern, its Michaelis constant and the effects of various inhibitors and high temperature. In B. evansiana, which requires SD treatment to form aerial tubers, the amount of IAA oxidase in leaves increased to a maximum after 2 days of SD treatment. When SDs with interrupted night were given, a maximum was reached after 6 SDs. Thus, the change in IAA oxidase content had an inverse correlation to that for IAA content observed previously.

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