Change in Immunoreactive Human Hepatic Triglyceride Lipase (HTGL) Mass and the Shelf‐Life of the HTGL ELISA Kit in Long‐Term Storage

Abstract

Objectives of this work are to study changes in the immunoreactive HTGL mass during storage under various conditions. In addition, the shelf‐life of the HTGL ELISA kit was confirmed. The immunological reactivity of HTGL in PHP stored in the liquid, frozen, or lyophilized state was monitored using purified human PHP‐HTGL as the standard material. Furthermore, the long‐term stability of the HTGL ELISA kit was ascertained. The immunoreactive HTGL mass in the lyophilized PHP maintained its initial immunological reactivity for at least 26 months at 4°C or lower. The other reagents included in the HTGL ELISA kit also have a long shelf‐life when they are stored at 4°C or less. HTGL in PHP was stabilized by lyophilization and can be used as the standard material for HTGL ELISA; the HTGL ELISA kit has a long shelf‐life, i.e., more than two years.