Chamomile decoction extract inhibits human neutrophils ROS production and attenuates alcohol-induced haematological parameters changes and erythrocytes oxidative stress in rat.

Mohamed-Amine Jabri,Jamel El-Benna,Lamjed Marzouki,Kais Rtibi,Mohsen Sakly,Mamane Sani,Hichem Sebai

doi:10.1186/s12944-016-0233-4

Abstract

BackgroundThe aim of this study was to evaluate the protective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against stimulated neutrophils ROS production as well as ethanol (EtOH)-induced haematological changes and erythrocytes oxidative stress in rat.MethodsNeutrophils were isolated and ROS generation was measured by luminol-amplified chemiluminescence. Superoxide anion generation was detected by the cytochrome c reduction assay. Adult male wistar rats were used and divided into six groups of ten each: control, EtOH, EtOH + various doses of CDE (25, 50, and 100 mg/kg, b.w.), and EtOH+ ascorbic acid (AA). Animals were pre-treated with CDE extract during 10 days.ResultsWe found that CDE inhibited (P ≤ 0.0003) luminol-amplified chemiluminescence of resting neutrophils and N-formyl methionylleucyl-phenylalanine (fMLF) or phorbolmyristate acetate (PMA) stimulated neutrophils, in a dose-dependent manner. CDE had no effect on superoxide anion, but it inhibited (P ≤ 0.0004) H2O2 production in cell free system. In vivo, CDE counteracted (P ≤ 0.0034) the effect of single EtOH administration which induced (P < 0.0001) an increase of white blood cells (WBC) and platelets (PLT) counts. Our results also demonstrated that alcohol administration significantly (P < 0.0001) induced erythrocytes lipoperoxidation increase and depletion of sulfhydryl groups (−SH) content as well as antioxidant enzyme activities as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). More importantly, we found that acute alcohol administration increased (P < 0.0001) erythrocytes and plasma hydrogen peroxide (H2O2), free iron, and calcium levels while the CDE pre-treatment reversed increased (P ≤ 0.0051) all these intracellular disturbances.ConclusionsThese findings suggest that CDE inhibits neutrophil ROS production and protects against EtOH-induced haematologiacal parameters changes and erythrocytes oxidative stress. The haematoprotection offered by chamomile might involve in part its antioxidant properties as well as its opposite effect on some intracellular mediators such as H2O2, free iron, and calcium.

Highlights

The aim of this study was to evaluate the protective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against stimulated neutrophils Reactive oxygen species (ROS) production as well as ethanol (EtOH)-induced haematological changes and erythrocytes oxidative stress in rat
Effect of CDE on luminol-amplified chemiluminescence in human neutrophils To investigate the antioxidant effect of CDE on human neutrophils, we first looked at the luminol-amplified chemiluminescence stimulated with phorbolmyristate acetate (PMA) (Fig. 1a) and fMLF (Fig. 1b) in these cells
Effect of CDE on fMLF and PMA-induced neutrophils superoxide anion production we focused on the experimental production of superoxide anion in human neutrophils treated with

Summary

Introduction

The aim of this study was to evaluate the protective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against stimulated neutrophils ROS production as well as ethanol (EtOH)-induced haematological changes and erythrocytes oxidative stress in rat. The enhanced ROS production may lead to oxidative stress and oxidation of vital cellular components, which induce cellular damage and cell death [4]. To protect cells against these harmful species we use a synthetic or natural antioxidants molecules [8, 9]. These latter are able to scavenge ROS and to up-regulate endogenous antioxidant defense systems [10]. Mammalian erythrocytes are endowed with extraordinarily efficient enzymatic and non-enzymatic antioxidant defense systems that act as ROS scavengers to limit their imposed damage [11]. Erythrocytes are considered as passive ‘reporter cells’ for the oxidative status of the whole organism and an increasing amount of attention is being paid to the use of plant molecules such as polyphenolic and carotenoid components [13] in the prevention and cure of various [13]

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