Challenges in the Next-Generation Sequencing Field

Abstract

The next-generation sequencing field has developed at such a rapid pace that the achievements of today exceed the challenges and limitations of the last few years. We have previously described how next-generation sequencing has advanced DNA sequencing from low throughput to high throughput and minimized labor-intensive practices to more automated processes. In this chapter, we will compare next-generation sequencing with the traditional Sanger method to demonstrate the remarkable recent developments in relation to improved speed, throughput, and accuracy. Lastly, we will provide an overview of lingering challenges that are still faced by next-generation sequencing.