Abstract
Selected reaction monitoring mass spectrometry (SRM-MS) has been developed enormously over the past two decades as a powerful technique to efficiently determine target protein concentration across many biological/clinical samples in terms of specificity, reproducibility, multiplexing capability, and accuracy. However, measurement of very low-abundance proteins in biofluids is still a challenge. Front-end sample processing can improve the sensitivity but only with the expense of assay variability. Recent technical development of MS instrument has improved sensitivity and selectivity of SRM assays. Scheduled SRM has improved overall multiplexing capability. Recent study has found inter- and intralaboratory SRM assay reproducible even with triple quadrupole/quadrupole trap from multiple vendors. Public repositories of targeted proteomics data have been growing rapidly. New sample preparation methodology and especially, LC and MS instrument development, will revolutionize SRM assay capability for larger-scale in-depth biological and clinical applications in future.
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