Chalcone Synthase: Method Optimization of Extraction and Activity Assay in Strawberry Fruit

Linda Zoungrana,Alyssa N Smith,M Cecilia Do Nascimento Nunes

doi:10.14293/s2199-1006.1.sor-.ppcyrqc.v1

Linda Zoungrana, Alyssa N Smith + Show 1 more

Open Access

https://doi.org/10.14293/s2199-1006.1.sor-.ppcyrqc.v1

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Publication Date: Jul 28, 2020

License type: CC BY 4.0

Affiliation: University of South Florida

Abstract

Method development and optimization play a central role in analytical chemistry and more specifically in food biochemistry. When it comes to research, it is common that analytical methods need to be modified to specific experimental biological tissues. While there are several published works on the activity of the enzyme chalcone synthase (CHS) in plant materials, such as sweet basil using ultra- high-performance liquid chromatography, there is a lack of information regarding extraction and activity of CHS in strawberries. Therefore, the main objective of this work was to optimize existing published methods for extraction and activity of CHS in strawberries, using spectrophotometric analysis. It was done through a literature search, a method dissection was performed, followed by theoretical optimization of the protocol, and finally an experimental optimization

Highlights

Chalcone synthase is a ubiquitous enzyme in higher plants
It catalyzes the sequential decarboxylative addition of three acetate units from malonyl-CoA to a p-coumaroyl-CoA starter molecule deriving from phenylalanine through the phenylpropanoid pathway[1], which is a rich source of metabolites in plants[2]
While there are several published works on the activity of the enzyme chalcone synthase (CHS) in plant materials, such as sweet basil using ultra- high-performance liquid chromatography, there is a lack of information regarding extraction and activity of CHS in strawberries

Summary

Introduction

Chalcone synthase is a ubiquitous enzyme in higher plants. While there are several published works on the activity of the enzyme chalcone synthase (CHS) in plant materials, such as sweet basil using ultra- high-performance liquid chromatography, there is a lack of information regarding extraction and activity of CHS in strawberries. From the literature search we based our method optimization on the work published by Ghasemzadeh et al (2016) on sweet basil using spectrophotometry and ionic exchange reasin rather than HPLC. This protocol was analyzed and adjusted for strawberry fruit

Objectives

Methods