外源cGMP调控盐胁迫下黑麦草种子萌发机制

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 外源cGMP调控盐胁迫下黑麦草种子萌发机制 DOI: 10.5846/stxb201801140105 作者: 作者单位: 甘肃农业大学生命科学技术学院,甘肃农业大学,甘肃农业大学生命科学技术学院,甘肃农业大学生命科学技术学院,甘肃农业大学生命科学技术学院,甘肃农业大学,甘肃农业大学生命科学技术学院,甘肃农业大学生命科学技术学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然基金项目（31560663） Exogenous cGMP regulates seed germination of ryegrass under salt stress Author: Affiliation: College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou,Gansu Agricultural University,College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou,College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou,College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou,Gansu Agricultural University,College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou,College of life science and technology,Gansu Agricultural University,;Gansu,Lanzhou Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:cGMP（cyclic guanosine-3'，5'-monophosphate）是一类环化核苷酸，能响应植物非生物胁迫的信号分子。以cGMP的膜透性类似物8-Br-cGMP为供体，研究cGMP对盐胁迫下黑麦草种子萌发及生理指标的影响。通过计算萌发指标、测定种子萌发过程中各项生理指标，探讨cGMP对黑麦草种子萌发时耐盐机制的作用。结果表明：低浓度（50 mmol/L）盐胁迫能促进黑麦草种子的萌发，随着盐浓度（100、150、200、250 mmol/L）的增加黑麦草种子的萌发率逐渐降低甚至抑制其萌发。而加入20 μmol/L cGMP能缓解100 mmol/L NaCl胁迫对黑麦草种子造成的伤害，使黑麦草种子的发芽率、发芽势、发芽指数、活力指数分别提高了7.4%、133.3%、52.1%、104.2%。此外，研究发现加入20 μmol/LcGMP能够促进100 mmol/L NaCl盐胁迫下黑麦草萌发期根的生长，比单独100 mmol/L NaCl盐胁迫处理下的黑麦草植株全长、根长、叶长分别提高了2.5、2.8倍和2.6倍，种子在萌发过程中可溶性糖、可溶性蛋白含量、淀粉酶活性，脯氨酸含量分别提高了47.9%、15.7%、94.3%、117.4%，而淀粉含量、MDA含量、电导率，O2-产生速率分别降低了40.9%、128.7%、88.6%、211.9%。说明20 μmol/LcGMP通过提高可溶性糖，可溶性蛋白含量及脯氨酸含量，同时减少MDA、O2-的产生，以此缓解盐胁迫对黑麦草种子的伤害、同时促进淀粉水解，从而加速种子萌发。 Abstract:cGMP (cyclic guanosine-3',5'-monophosphate) is a type of cyclic nucleotide and signaling molecule that can be responsive to plant abiotic stress. Using cGMP membrane permeability analog 8-Br-cGMP as a donor, this study investigated the effects of cGMP on seed germination and physiological indicators of ryegrass under salt stress. The effects of cGMP on the salt tolerance mechanism during the germination of ryegrass were determined through calculating the germination index and determining the physiological indicators in the process of seed germination. The results showed that a low concentration (50 mmol/L) of salt stress could promote the germination of ryegrass seeds. With increasing salt concentrations (100, 150, 200, and 250 mmol/L), the germination rate of ryegrass seeds gradually decreased and was even inhibited. Adding 20 μmol/L cGMP can relieve the damage caused by 100 mmol/L NaCl stress on ryegrass seed, increasing the germination rate, germination potential, germination index, and vigor index of ryegrass seed by 7.4%, 133.3%, 52.1%, and 104.2%, respectively. In addition, compared to the 100 mmol/L NaCl stress condition alone, the growth of roots of ryegrass could be promoted and the total length, root length, and leaf length of ryegrass plants increased, by 2.5, 2.8, and 2.6 times, respectively, when 20 μmol/L cGMP was added. The soluble sugar, soluble protein content, amylase activity, and proline content of seeds increased by 47.9%, 15.7%, 94.3%, and 117.4%, respectively, while starch content, MDA content, conductivity, and O2-. production rate were reduced by 40.9%, 128.7%, 88.6%, and 211.9%, respectively. The results showed that 20 μmol/L cGMP could increase the contents of soluble sugar, soluble protein, and proline, and reduce the production of MDA and O2-, so as to relieve the damage caused by salt stress on ryegrass seed and promote the hydrolysis of starch, which accelerated seed germination. 参考文献 相似文献 引证文献