Abstract
Colletotrichum gloeosporioides is the main causal agent of anthracnose in various plant species. Determining the molecular mechanisms underlying the pathogenicity and fungicide resistance of C. gloeosporioides could help build new strategies for disease control. The major facilitator superfamily (MFS) has multiple roles in the transport of a diverse range of substrates. In the present study, an MFS protein CgMFS1 was characterized in C. gloeosporioides. This protein contains seven transmembrane domains, and its predicted 3D structure is highly similar to the reported hexose transporters. To investigate the biological functions of CgMFS1, the gene knock-out mutant ΔCgMFS1 was constructed. A colony growth assay showed that the mutant was remarkably decreased in vegetative growth in minimal medium supplemented with monosaccharides and oligosaccharides as the sole carbon sources, whereas it showed a similar growth rate and colony morphology as wild types when using soluble starch as the carbon source. A stress assay revealed that CgMFS1 is involved in oxidative stress but not in the fungicide resistance of C. gloeosporioides. Furthermore, its pathogenicity was significantly impaired in the mutant, although its appressorium formation was not affected. Our results demonstrate that CgMFS1 is required for sugar transport, resistance to oxidative stress, and the pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.
Highlights
The genus Colletotrichum, which comprises about 600 species, is one of the most common and notorious phytopathogens
A gene encoding an major facilitator superfamily (MFS) protein was identified from the genome of C. gloeosporioides, and the gene was called CgMFS1
1185-bp open reading frame separated by four small introns, and it encodes a polypeptide of 394 amino acids (Supplementary Sequence Information)
Summary
The genus Colletotrichum, which comprises about 600 species, is one of the most common and notorious phytopathogens. Members of the genus attack a variety of crops throughout the world, which leads to heavy economic losses [1]. Most Colletotrichum species have a hemibiotrophic lifestyle, which comprises a biotrophic phase at the early infection stage and a necrotrophic phase later on [2]. Among these species, Colletotrichum gloeosporioides can cause anthracnose diseases in both aerial plant parts and in the postharvest fruits of many important economic crops [3]. Synthetic fungicides have been widely used to control anthracnose in H. brasiliensis; the indiscriminate use of synthetic fungicides can lead to pathogens becoming fungicide resistance and can cause environmental contamination. Clarifying the molecular pathogenicity and fungicide resistance mechanisms of C. gloeosporioides might provide new clues for controlling anthracnose
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