Abstract
Concentrated growth factor (CGF) membranes are widely used in basic and clinical research of soft and hard tissue regeneration, but its effect on periodontal tissue regeneration is less studied. This study explored the role of CGF membranes in periodontal tissue regeneration mediated by human umbilical cord mesenchymal stem cells (hUCMSCs). HUCMSCs and human periodontal ligament fibroblasts (HPLFs) were extracted and identified by microscope and flow cytometry. The effects of the extracted CGF membrane on cell viability, osteogenic differentiation ability, osteopontin (OPN) expression, alkaline phosphatase (ALP) content, and osteogenic differentiation-related genes (Runt-related transcription factor 2 (RUNX2); osteocalcin (OCN); ALP), Tafazzin (TAZ) expression, and nuclear transfer were examined by MTT assay, alizarin red staining, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Rescue experiments were performed to examine the effects of TAZ transfection and cell coculture. In the identified hUCMSCs (positive expressions of CD29, CD44, CD146, and CD105), overexpressed TAZ (pc-TAZ) enhanced the promotive effect of CGF membrane on cell viability, cell cycle, mineralization, ALP content and expressions of OPN, TAZ and osteogenic differentiation-related genes, and nuclear transfer. However, silencing TAZ showed opposite effects. The coculture of hUCMSCs and HPLFs further promoted the basic biological functions of HPLFs by upregulating osteogenic differentiation-related genes and COL-1 but downregulated MMP1 expression. Pc-TAZ could enhance the effect of CGF membrane on promoting periodontal tissue regeneration. CGF membrane promoted periodontal tissue regeneration through upregulating TAZ and osteogenic differentiation-related genes.
Highlights
Teeth are the hardest organs in the human body, but periodontal tissues are vulnerable to external stimuli under the influence of various external factors
By employing MTT assay, alizarin staining, immunofluorescence staining, and enzyme-linked immunosorbent assay (ELISA) assay, we investigated the effect of Concentrated growth factor (CGF) fibrin membranes on human umbilical cord mesenchymal stem cells (hUCMSCs)
Periodontal pockets contained a large amount of inflammatory mediators, especially those related to chronic inflammation, such as tumor necrosis factor α, interleukin, and prostaglandin, which could enter the blood circulation system from periodontal pockets and cause systemic inflammatory reactions [26, 27]
Summary
Teeth are the hardest organs in the human body, but periodontal tissues are vulnerable to external stimuli under the influence of various external factors. Lesions in the periodontal tissues could cause periodontitis, leading to the destruction and absorption of periodontal ligaments, cementum, alveolar bone, and other dental support tissues [1, 2]. The main periodontal treatment in clinical practice is to remove tartar, plaque, and diseased root surface to eliminate local inflammation of periodontal tissues and prevent further progression [3]. Tissue regeneration technology has become a promising strategy for the treatment of periodontal tissue lesions [4]. Since the guided tissue regeneration (GTR) technology was proposed in 1980, tissue regeneration engineering has attracted much research attention in the past ten years [5, 6]. Mesenchymal stem cells play an important role in maintaining tissue self-
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
