Abstract
The aim of this study was to establish the use of c-Fos immunoreactivity as a marker for functional mapping in the auditory system in response to direct electrical stimulation of the cochlear nerve in the cerebellopontine angle. In rats the cochlear nerve was electrically stimulated with a biphasic current (120–250 μA, 5 Hz) for 30 min using a bipolar concentric Tungsten electrode. Bilateral cochlectomy was performed in a control group in order to investigate basal expression of c-Fos in the auditory brainstem nuclei. The response of auditory brainstem nuclei to electrical stimulation and the completeness of cochlear ablations were electrophysiologically verified. After the experiments, the animals were prepared for cryotomy and c-Fos immunohistochemistry. The results were morphologically analyzed and statistically compared among groups. In anesthetized animals with unilateral electrical stimulation of the cochlear nerve increased expression of c-Fos was detected in the ipsilateral ventral (VCN) and bilateral dorsal cochlear nucleus (DCN), whereas the VCN of the contralateral side revealed only few immunoreactive cells. In animals with bilateral cochlear ablation the number of c-Fos reactive cell nuclei representing basal expression was generally low in the VCN and DCN of both sides. Our data show that electrical stimulation of the cochlear nerve leads to increased expression of c-Fos in the cochlear nucleus. It also confirms bilateral connections between the cochlear nuclei. These experimental results suggest that c-Fos immunoreactivity mapping provides a powerful tool for functional investigations on the cellular level after direct electrical stimulation of the cochlear nerve. Future functional studies analyzing the effect of electrical stimulation of the central auditory system as performed by auditory brainstem implants could be investigated in detail by mapping c-Fos expression on cellular level.
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