Abstract
Morphological and molecular changes produced byAutographa californicanuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), ofSpodoptera frugiperdaand a nonpermissive cell line, FPMI-CF-203 (CF-203), ofChoristoneura fumiferanaare described. CF-203 cells inoculated withAcMNPV showed a DNA ladder and morphological changes such as plasma membrane granulation, blebbing, and nuclear fragmentation, which are characteristic of apoptosis. Typical virus replication and occlusion body (OB) production were seen in SF-21 cells inoculated withAcMNPV and no apoptosis-like symptoms were observed. mRNA for the apoptosis suppressor gene p35 was detected 9 hr later inAcMNPV-inoculated CF-203 cells than in SF-21 cells. Only a trace amount of mRNA for theAcMNPV-inhibitor of apoptosis homologue (Ac-iap) gene and no mRNAs for the late genes,AcMNPV-polyhedrin (Ac-polh) andAcMNPV-p10 (Ac-p10), were detected inAcMNPV-inoculated CF-203 cells. Inoculation of CF-203 cells withCfMNPV at least 12 hr prior to inoculation withAcMNPV prevented apoptosis-like cell death, and mRNAs forAc-iap, Ac-polh,andAc-p10genes were expressed resulting in successful virus replication and OB production.
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