Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can potentially infect female reproductive organs. In this study, we investigated the presence of SARS-CoV-2 in cervicovaginal fluid. This study included 31 female patients aged 18–65 years. The presence of SARS-CoV-2 RNA was investigated by RT‒PCR in two separate cervicovaginal swab samples collected from patients 14 days apart. Viral RNA was extracted using Bio-Speedy ®vNAT® Viral Nucleic Acid Buffer (vNAT) solution, and SARS-CoV-2 RNA was analyzed using Bio-Speedy SARS-CoV-2 RT‒qPCR kits in a Bio-Rad CFX96 TouchTM device. First and second cervical swab samples were collected 14 days apart. The SARS-CoV-2 RNA result was negative in all 53 cervicovaginal swab samples collected. Negative SARS-CoV-2 RNA results in cervicovaginal swab samples indicate that coronavirus disease 2019 (COVID-19) is not sexually transmitted. However, the number of studies on this subject and the sample size examined are still insufficient for reaching this conclusion.
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