Abstract
Nicotinic acetylcholine receptors (nAChRs) are associated with various cancers, but the relation between nAChRs and cervical cancer remains unclear. Therefore, this study investigated the differential expression of nAChR subunits in human cervical cancer cell lines (SiHa, HeLa, and CaSki) and in normal ectocervical cell lines (Ect1/E6E7) at mRNA and protein levels. Two specific nAChR subtype blockers, αO-conotoxin GeXIVA and α-conotoxin TxID, were then selected to treat different human cervical cancer cell lines with specific nAChR subtype overexpression. The results showed that α3, α9, α10, and β4 nAChR subunits were overexpressed in SiHa cells compared with that in normal cells. α9 and α10 nAChR subunits were overexpressed in CaSki cells. α*-conotoxins that targeted either α9α10 or α3β4 nAChR were able to significantly inhibit cervical cancer cell proliferation. These findings may provide a basis for new targets for cervical cancer targeted therapy.
Highlights
Cervical cancer is one of the most common female malignancies in the world with 570,000 cases and 311,000 deaths globally in 2018 [1]
Polymerase Chain Reaction (PCR) amplification was performed to detect whether Nicotinic acetylcholine receptors (nAChRs) subunits were expressed in human amplification detect whether nAChR
Recent have shown that nAChRs are in in close diseases, Recent studies studies have shown that nAChRs are close relation relation with with tobacco-related tobacco-related diseases, especially cancer
Summary
Cervical cancer is one of the most common female malignancies in the world with 570,000 cases and 311,000 deaths globally in 2018 [1]. It poses a serious threat to women’s health, especially in less developed countries [2]. Persistent infection of high-risk human papillomavirus (HPV) is a major risk factor for cervical cancer [3,4]. HPV vaccines are able to effectively prevent infection, they cannot cure the identified infections [5]. The clinical treatments of cervical cancer are mainly based on surgical treatment, supplemented with chemotherapy and radiation therapy, which are usually accompanied by severe systemic toxicity and chemical resistance. With the continuous development of molecular biology, targeted therapy has attracted wide attention due to its high efficacy, specificity, and low adverse reactions. It has been applied to some tumors and produced a marked effect. The targeted therapy of cervical cancer is still in the basic research period, and finding an effective target is of great importance
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