Cerium protects adipose tissue derived mesenchymal stem cell from slow freezing thawing damage via affecting apoptosis genes

Abstract

IntroductionBecause of the important role of cerium in removal of free radicals we performed this study to investigate effect of different doses of cerium on freezing thawing damage of adipose tissue derived mesenchymal stem cells (MSC) via studying cellular viability and expression of apoptosis related genes. MethodsAn experimental study was conducted on MSCs derived from adipose tissue of mice. The isolated MSCs were approved by flowcytometry. There were two main groups of slow and rapid freezing. Each group was treated with cerium doses 0, 5, 10, 20 and 40 μg/ml. Cellular viability and gene expression were studied. The investigated genes were Fas, Bax, Bcl2, P53 and Caspase3. ResultsCellular viability was increased in slow freezing group with dose 5 μg/ml and in rapid freezing group with dose 10 μg/ml (P < 0.001). No significant difference was found for other doses. Bax expression was down regulated at all doses of show freezing group and doses 5–20 μg/ml of rapid freezing group. Fas expression was down regulated at doses 10 and 20 μg/ml of slow freezing group. Caspase3 was down regulated at doses 5 and 20 μg/ml of slow freezing group. There were some up regulations in slow and rapid freezing groups. ConclusionThe results showed that cerium at low doses may inhibit apoptosis in slow freezing samples. The results for rapid freezing samples were inconclusive. In general, low dose cerium is recommended for slow freezing. Using rapid freezing is not suggested.