Ceria Nanostructures as Biosensing Platform for Glucose Sensing

Abstract

Metal oxide semiconductors at the nanometric scale have attracted considerable attention in recent years. Here, cerium oxide nanostructures were prepared by electrodeposition technique on a fluorine doped tin oxide (FTO) substrate. The fabricated CeO2 samples were characterized by scanning electron microscopy (SEM), X-ray diffraction analysis (XRD) and UV-visible spectroscopy. Additionally, the CeO2 samples were tested for glucose sensing. The amperometric current response of the glucose biosensor was recorded at every addition of glucose. Furthermore, electrochemical impedance spectroscopic (EIS) method was used to study the charge transfer in the fabricated biosensors. Finally, a comparison of CeO2 control sample and the CeO2 samples fabricated in the presence of DMSO was carried out. The morphology controlled CeO₂ exhibits high sensitivity of 134 μA/cm². mM with a response time of < 2 s, wider linear range (0.1 – 3.0 mM), low detection limit of 0.3 μM and lower value of Michaelis–Menten constant, KM app, 0.041 mM.