Abstract

Amyotrophic lateral sclerosis (ALS) is a relentlessly progressive neurodegenerative disorder. As previous therapeutic trials in ALS have been severely hampered by patients’ heterogeneity, the identification of biomarkers that reliably reflect disease progression represents a priority in ALS research. Here, we used the D50 disease progression model to investigate correlations between cerebrospinal fluid (CSF) neurofilament light chain (NfL) levels and disease aggressiveness. The D50 model quantifies individual disease trajectories for each ALS patient. The value D50 provides a unified measure of a patient’s overall disease aggressiveness (defined as time taken in months to lose 50% of functionality). The relative D50 (rD50) reflects the individual disease covered and can be calculated for any time point in the disease course. We analyzed clinical data from a well-defined cohort of 156 patients with ALS. The concentration of NfL in CSF samples was measured at two different laboratories using the same procedure. Based on patients’ individual D50 values, we defined subgroups with high (<20), intermediate (20–40), or low (>40) disease aggressiveness. NfL levels were compared between these subgroups via analysis of covariance, using an array of confounding factors: age, gender, clinical phenotype, frontotemporal dementia, rD50-derived disease phase, and analyzing laboratory. We found highly significant differences in NfL concentrations between all three D50 subgroups (p < 0.001), representing an increase of NfL levels with increasing disease aggressiveness. The conducted analysis of covariance showed that this correlation was independent of gender, disease phenotype, and phase; however, age, analyzing laboratory, and dementia significantly influenced NfL concentration. We could show that CSF NfL is independent of patients’ disease covered at the time of sampling. The present study provides strong evidence for the potential of NfL to reflect disease aggressiveness in ALS and in addition proofed to remain at stable levels throughout the disease course. Implementation of CSF NfL as a potential read-out for future therapeutic trials in ALS is currently constrained by its demonstrated susceptibility to (pre-)analytical variations. Here we show that the D50 model enables the discovery of correlations between clinical characteristics and CSF analytes and can be recommended for future studies evaluating potential biomarkers.

Highlights

  • Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder that is predominately characterized by the progressive loss of motor neuron function

  • Rate (Tortelli et al, 2012; Lu et al, 2015; Menke et al, 2015; Steinacker et al, 2016, 2018b; Gaiani et al, 2017; Poesen et al, 2017; Andres-Benito et al, 2018; Gong et al, 2018; Rossi et al, 2018; Scarafino et al, 2018; Schreiber et al, 2018; Abu-Rumeileh et al, 2020). These findings suggest that Cerebrospinal fluid (CSF) neurofilament light chain (NfL) concentrations at baseline may allow early stratification of patients in clinical trials according to anticipated progressiveness, thereby reducing clinical heterogeneity and enabling the detection of significant treatment effects even in smaller ALS patient cohorts

  • Cerebrospinal fluid Log[NfL] levels were significantly higher in the ALS group as compared to the non-neurological control, disease control, and ALS mimic groups

Read more

Summary

Introduction

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder that is predominately characterized by the progressive loss of motor neuron function. As phenotypic variability and disease course variability represent major constraints to clinical management and therapeutic trials in ALS, the search for biomarkers that can accurately predict progression is a current research priority. The detection of significant treatment effects in these trials requires large sample sizes and consumes time and resources, which could be improved by specific pharmacodynamic or prognostic/predictive biomarkers. The importance of such biomarkers has been underlined in the recently revised Airlie House consensus criteria for clinical trial development in ALS (Van Den Berg et al, 2019)

Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call