Cerebrospinal fluid levels of 8‐isoprostane, a marker of oxidative stress, are elevated in patients with Alzheimer’s disease compared to healthy subjects: Does the malfunction in the cell’s powerhouse lead to Alzheimer’s disease?

Abstract

AbstractBackgroundOxidative stress may play an important role in the pathology of Alzheimer’s disease (AD). 8‐Isoprostane is a prostaglandin (PG)‐F2‐like compound belonging to the F2 Isoprostane class that is produced in vivo by the free radical‐catalyzed peroxidation of cells membrane arachidonic acid. 8‐Isoprostane is a biomarker of oxidative stress, and could possibly be a surrogate marker of the mitochondrial health in AD.MethodWe measured concentrations of cerebrospinal fluid (CSF) 8‐isoprostane from 113 subjects ranging from healthy subjects (HS, n=25) , to Mild Cognitive Impairment with positive AD biomarkers (MCI+, n=16), AD( n=40), and non‐AD dementia (non‐AD, n=32). Analysis was done using 8‐isoprostane Enzyme‐linked immunosorbent assay from Cayman Chemicals (Cayman Chemicals, MI, USA).ResultPatient characteristics can be seen in Table 1. Analyzing the CSF levels of 8‐isoprostane, a significant difference between 8‐isoprostane concentrations among the groups (ANOVA) was found. Post‐hoc test (t‐test) revealed a difference between the HS and AD groups (Figure 1).ConclusionIn conclusion 8‐isoprostane may be a good surrogate marker of cellular oxidative stress and may be used to evaluate mitochondria’s role in AD pathology in vivo.