Abstract
The aim of the present study was to adapt a commercial kit anti-HSV IgG used for serum samples to be performed with a CSF sample. Forty CSF specimens from 38 patients with suspected CNS HSV infection were serially diluted for detecting anti-HSV IgG by enzyme immunoassay (EIA). The same samples were also analyzed with the polymerase chain reaction (PCR). The sensitivity of EIA test for HSV was 5% (dilution 1:40) and 65% (dilution 1:2) in CSF, and HSV DNA PCR was 15%. The combined analysis of EIA (dilution 1:2) and PCR increased the sensitivity up to 72.5%. The inflammatory CSF was associated with positive HSV PCR. We demonstrated the importance to adapt serological anti-HSV IgG EIA test for CSF assays to increase the accuracy of the analysis, considering the low concentration of specific antibodies in CSF.
Highlights
Herpes simplex virus (HSV) is a cause of a severe disease of the central nervous system (CNS) in humans
The aim of this study was to adapt a commercial kit anti-HSV IgG used for serum samples to be performed with cerebrospinal fluid (CSF) samples, increasing the accuracy of the laboratory diagnosis of HSV infection in the CNS
Repeatability assay with twenty replicates on the same day and reproducibility assay with triplicates over the course of 20 days were performed with the pool of CSF
Summary
Herpes simplex virus (HSV) is a cause of a severe disease of the central nervous system (CNS) in humans. Results: The sensitivity of EIA test for HSV was 5% (dilution 1:40) and 65% (dilution 1:2) in CSF, and HSV DNA PCR was 15%. Conclusions: We demonstrated the importance to adapt serological anti-HSV IgG EIA test for CSF assays to increase the accuracy of the analysis, considering the low concentration of specific antibodies in CSF. Metodos: Quarenta amostras de LCR de 38 pacientes com suspeita de infecção por HSV no SNC foram diluídas para pesquisa de anticorpos anti-HSV IgG pelo método imunoenzimático (EIA). Resultados: A sensibilidade do teste EIA para o HSV consistiu em 5% (diluição 1:40) e 65% (diluição 1:2) no LCR, e o PCR do DNA do HSV, 15%. Conclusões: Demonstramos a importância na adaptação previa do teste sorológico anti-HSV IgG EIA para ensaios do no LCR, a fim de aumentar a acuracia da análise, considerando a baixa concentração de anticorpos específicos no LCR. Both viruses (HSV-1 and HSV-2) can trigger a broad spectrum of neurological manifestations, Samya Jezine DA SILVA https://orcid.org/0000-0003-1978-0548; Mauro Jorge CABRAL-CASTRO https://orcid.org/0000-0002-1755-999X; Maria Angélica GUIMARÃES https://orcid.org/0000-0002-5364-7831; José Mauro PERALTA PUCCIONI-SOHLER https://orcid.org/0000-0001-6007-9142 https://orcid.org/0000-0002-2615-4820; Marzia
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