Cerebellar electric activity in anaesthesia and in chronic mild stress

Abstract

Event Abstract Back to Event Cerebellar electric activity in anaesthesia and in chronic mild stress Jelena Podgorac1*, Gordana Stojadinovic1, Milka Culic1, Ljiljana Martac1, Goran Kekovic1 and Slobodan Sekulic2 1 University of Belgrade, Institute for Biological Research, Russia 2 University of Novi Sad, Medical Faculty, Russia Background and Aim: It is hypothetized that cerebellum could reduce performance variability in order to achieve synchronization between the expected sensory consequences of action or cognition and actual sensory feedback (1). Our aim is to investigate cerebellar local field potentials in various states of anaesthesia and in the rodent model of mild stress. Methods: The experiments were performed on adult Wistar male rats. One group of animals was exposed to specific anaesthetic (nembutal or ketamine) and the other group was exposed to chronic mild stress. Local field potentials of cerebellar vermal cortex and cerebral parietal cortex were simultaneously recorded in acute experiments on rats of the first group and after 10 days of isolation in chronically implanted rats of the second group. After amplification, filtration and analog to digital conversion of biosignals, spectral entropy of cerebellar activity for each of five defined frequency ranges - delta, theta, alpha, beta and gamma, was calculated (2). Results: Spectral entropy, as a measure of activity, in the case of cerebellum had lower values than the spectral entropy of cerebrum in low frequency ranges, regardless of the type of applied anaesthetic. Various anaesthetics evoked different effects on spectral entropy of cerebellar electrocortical activity: spectral entropy of delta range greatly dominated under nembutal anaesthesia, while ketamine appeared to affect the spectral entropy of higher frequency ranges. In the second group of animals exposed to mild stress the spectral entropy of cerebellar activity in higher frequency ranges had greater values than under particular anaesthesia. Conclusion: Changes in cerebellar electric activity may indicate that cerebellum could have a role in coordinating activity as animals/humans progress from sleep, anaesthesia, towards full wakefulness and high attention in various pathophysiological states. Acknowledgements: This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia (project 143021).