Cereal viruses – brome mosaic virus: prevalence and diagnostics

Abstract

Analysis of the prevalence and bioecological characteristics of the brome mosaic virus (BMV), which is a widespread polyphage virus that causes significant economic damage to cereals. Experimental studies were carried out in the laboratory of the All-Russian Science-Research Institute of plant Quarantine (Moscow region), visual examinations of crops and sampling of winter wheat in the tillering phase were carried out in the farms of the Rostov region, the Chechen Republic, in the Moscow region (Russian State Agrarian University -Moscow Timiryazev Agricultural Academy) in 2019- 2020. Route surveys of fields in each region were carried out in the spring. The visually estimated prevalence of viroses on average did not exceed 0.5%. The detection and identification of the brome mosaic virus in plants with symptoms of mosaic and mottling was carried out by the method of classical PCR with visualization of the results by gel electrophoresis. Brome mosaic virus was detected by RT-PCR in winter wheat samples from the Rostov region. No brome mosaic virus was detected in winter wheat plants from the Chechen Republic, Moscow region, although the leaves showed virus-like symptoms.