CeO2 Nanoparticles Effect on Acute Hemorrhagic Shock Induced-hepatic Stress Injury in a Mouse Model.

Abstract

To explore the effects of CeO2 nanoparticles on liver injury, inflammation, oxidative stress, and coagulation function in hemorrhagic shock (HS)-induced hepatic stress injury. Experimental study. Study Place and Duration: Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, China, from March 2017 to July 2020. HS mice were treated with different doses of CeO2 nanoparticles suspension (1 mg/ml), 0.1 ml/100 g, 0.2 ml/100 g, 0.5 ml/100 g. Levels of NF-κB, IFN-γ, IL-1β, IL-6, and TNF-α in tissue homogenate were measured by ELISA. Oxidative stress-related factors in liver tissue homogenate were also evaluated. Coagulation function was determined by measurement of the prothrombin time (PT) and activated partial thromboplastin time (APTT) as well as using thromboelastography analysis. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected for liver function. Survival analysis within 24 hours after the model establishment was conducted by K-M curve. Hemorrhagic shock resulted in obvious tissue hemorrhage, elevated levels of AST and ALT, and significantly shorter survival for HS mice, which was improved by 0.5 ml/100 g CeO2 nanoparticles suspension. Treatment of CeO2 nanoparticles significantly decreased HS-induced inflammation, oxidative stress, and coagulation function in a dose-dependent manner. CeO2 nanoparticles could suppress inflammation and oxidative stress, as well as improve liver and coagulation function in hemorrhagic shock-induced hepatic stress injury mice. CeO2 nanoparticles, Hemorrhagic shock-induced hepatic stress injury, Inflammatory response, Oxidative stress, Coagulation function.