Century-long recovery of mycorrhizal interactions in European beech forests after mining

Abstract

Abstract Background and aims Ecological restoration strategies are emerging globally to counteract biodiversity loss and ecosystem degradation. However, restored ecosystems may not reach undisturbed biodiversity and functionality. One reason of this limited success may be a focus on short-term recovery of diversity, composition, or isolated functions. These simplified metrics may underestimate the real time ecosystems need to recover. Thus, studies of more complex metrics, like biotic interactions, at larger timescales, are essential to understand ecosystem recovery. Methods Using molecular identification, we assessed the recovery of the interactions between ectomycorrhizal (EcM) fungi and European beech (Fagus sylvatica L.) in two opencast iron mines in use since the fourteenth century and abandoned over 107 and 148 years. Results Species richness, species diversity, Basidiomycota/Ascomycota abundance ratio and taxonomic distinctness of EcM fungi recovered to undisturbed values, whereas species composition was still different. Certain fungal functional traits (i.e. exploration and sporocarp types) also reached undisturbed values. Differences in soil pH and NH4+ affected the composition of the EcM communities associated with beech, suggesting that mining caused a long-term impact in soil biogeochemistry, that directly impacted beech-EcM interactions. Conclusion Mycorrhizal interactions require more than 150 years to recover following mining. Contrary to the rapid recovery response provided by simple metrics like species richness, recovery metrics with more ecological information, like the identity of plant-EcM interactions, may be still capturing signals of incomplete recovery.