Abstract
ABSTRACTThe centrosome is the organizing center of microtubules in the cell, the basis for the origin of cilia and flagella and a site for the concentration of a regulatory proteins multitude. The centrosome comprises two centrioles surrounded by pericentriolar material. Centrioles in the cells of different organisms can contain nine triplets, doublets or singlets of microtubules. Here, we show that in somatic cells of male wasp larvae Anisopteromalus calandrae, centrioles do not contain microtubules and are composed of nine electron-dense prongs, which together form a cogwheel structure. These microtubule-free centrioles can be the platform for procentriole formation and form microtubule-free cilia-like structures. In nymph and imago cells centrioles have a microtubule triplet structure. Our study describes how centriole structure differs in a development-stage-dependent and a cell-type-dependent manner. The discovery of a centriole without microtubules casts a new light on the centriole formation process and the evolution of this organelle.
Highlights
The ultrastructure of centrioles was described for the first time in the middle of the 1950s, when the arsenal of cell biology methods was enhanced by electron microscopy (Fawcett and Porter, 1954; Burgos and Fawcett, 1955; Bernhard and de Harven, 1956)
Ultrastructure of male centrioles in three species of wasps We investigated cells of three species of wasps: Cotesia congregata, Nasonia vitripennis and A. calandrae
We found that in cells of A. calandrae larvae, centrioles can form structures that are analogous to primary cilia
Summary
The ultrastructure of centrioles was described for the first time in the middle of the 1950s, when the arsenal of cell biology methods was enhanced by electron microscopy (Fawcett and Porter, 1954; Burgos and Fawcett, 1955; Bernhard and de Harven, 1956). In present study it has been shown that the structure of centrioles at different stages of development of the organism can differ even more dramatically than previously shown for either Drosophila melanogaster and C. elegans; centrioles in the insect Anisopteromalus calandrae larvae did not contain MT.
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