Centre for Disease Control and Prevention (CDC) bottle bioassay: A real complementary method to World Health Organization (WHO) susceptibility test for the determination of insecticide susceptibility in malaria vectors

Abstract

In order to investigate the complementarities between both World Health Organization (WHO) and Centre for Disease Control and Prevention (CDC) methods and the specificities of each method useful for the determination of the insecticide susceptibility in malaria vectors, larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Littoral department. WHO susceptibility tests were conducted on unfed female mosquitoes aged 2 to 5 days old with impregnated-papers with deltamethrin (0.05%) and bendiocarb (0.1%), whereas CDC susceptibility tests were conducted with stock solutions of deltamethrin and bendiocarb (12.5 µg per bottle). WHO method and CDC method gave comparable results. There were complementarities between both methods. Both WHO and CDC methods are two complementary tools for the determination of insecticide susceptibility in malaria vectors. However, each method has also its own specificity. Key words: Complementary tools, insecticide, resistance, malaria vectors.