Central interleukin-1β-induced inhibition of acid secretion in rats: Specificity of action

Abstract

It is well established that IL-1β acts in the brain to potently inhibit gastric acid secretion in pylorusligated rats. The present study was designed to further investigate the specificity and mechanisms of the centrally mediated antisecretory action of IL-1β in conscious rats. Intracerebroventricular injection of IL-1β (100 ng) decreased acid secretion in pylorus-ligated rats and inhibited basal and pentagastrin-stimulated acid secretion in rats with chronic gastric fistula. The antisecretory effect of IL-1β (100 ng) injected into the lateral ventricle of pylorus ligated rats was completely reversed by prior intracerebroventricular injection of the IL-1 receptor antagonist, IL-1ra, (100 μg). Peripheral administration of the somatostatin monoclonal antibody, CURE.S6, did not modify intracisternal IL-1β-induced inhibition of acid secretion in pylorus ligated rats. IL-6 and tumor necrosis factor-α (100 ng) injected intracisternally did not influence gastric acid secretion in pylorus-ligated rats. These data show that IL-1β action in the CNS is mediated through interaction with specific IL-1 receptors and is selective to this cytokine. IL-1β antisecretory action can be observed under basal and pentagastrin-stimulated conditions and is independent from somatostatin release in the periphery.