Central corticosterone disrupts behavioral and neuroendocrine responses during lactation

Abstract

Administration of a high dose of chronic peripheral corticosterone during the postpartum period has been shown to lead to reduced maternal care, but the interference of acute corticosterone, mimicking a situation of acute stress, on maternal behavior has not been well established. Therefore, the aim of our study was to investigate the influence of acute central corticosterone on behavioral and neuroendocrine responses during lactation. On day 7 of lactation, female rats were treated with vehicle (5μL; i.c.v.) or corticosterone (10ng/5μL; i.c.v.) 30 min before the start of the experiment. To evaluate maternal behavior, the pups were returned to the side of their home cages opposite the previous nest, and the resulting behavior of the lactating rats was filmed for the next 30min. Plasma levels of oxytocin and the amount of milk consumed by the pups were evaluated 15min after the onset of suckling. In addition, the double-labeled c-Fos/oxytocin neurons in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) and c-Fos immunoreactivity in medial preoptic area (MPOA) neurons were quantified for each lactating rat. Corticosterone decreased maternal care, plasma oxytocin levels, milk consumption by the pups, the activation of oxytocinergic neurons in hypothalamic nuclei, and c-Fos immunoreactivity in MPOA neurons. Our results indicate that changes in the behavioral responses of lactating rats treated with corticosterone may be related to disruption of the neuroendocrine control of oxytocin secretion.