Central action of nitric oxide in the saltwater-acclimated duck: modulation of extrarenal sodium excretion and vasotocin release

Abstract

Hypothalamic nuclei close to the third ventricle (V III) represent key structures in avian osmoregulation concerned with the control of salt gland activity and release of the antidiuretic hormone [Arg 8]vasotocin (AVT). Nitric oxide (NO) acting as a paracrine transmitter in the hypothalamus has been shown to contribute to the maintenance of salt and fluid balance in mammals. The saltwater-acclimated duck was used in the present study as a well-characterized osmoregulatory model to investigate the role of central NO in hypothalamic perception or integration of osmoregulatory signals in marine birds. During osmotically induced steady-state salt gland secretion, the V III of conscious ducks was microperfused with artificial cerebrospinal fluid (aCSF) alone, aCSF containing the NO-donor SNAP or the peptide [Val 5]angiotensin II (ANGII) and alterations in salt gland activity, arterial pressure and the release of AVT were continuously monitored. No changes occurred during intracerebroventricular microperfusion with aCSF. Central application of ANGII, a known inhibitory hypothalamic transmitter in the control of salt gland function, markedly blocked salt gland osmolal excretion. Central stimulation with the NO-donor SNAP significantly reduced osmolal excretion from 0.41±0.02 to 0.22±0.04 mosmol/min. Both ANGII and SNAP caused a rise in plasma AVT at either slightly elevated (ANGII) or constant (SNAP) arterial pressure. Employing NADPH-diaphorase histochemistry in the duck hypothalamus to localize sites of NO synthesis, periventricular neurons, nerve fibers in close association to the V III and also parvocellular neurons of the paraventricular nucleus could be labeled. These data suggest a modulatory role for hypothalamic NO within the central osmoregulatory circuitry controlling salt gland function and AVT release in marine birds.