Abstract
The aim of this study was to examine the anti-proliferation and anti-migration effect of Centipede Scolopendra extracts (CSE) on human epidermoid carcinoma cells (high-EGFR expression) A431 and elucidate the underlying signaling mechanisms. MTT and colony formation assays were used. Migration and invasion potential of A431 cells were examined by wound-healing assays and matrigel invasion chamber assays. To investigate the underlying molecular mechanisms, we used ELISA to analyze the expression of EGF, Western blotting to analyze the expression of MMP2 (matrix metalloproteinase 2), MMP9 and EGFR, PCR to analyze the mRNA expression of EGFR pretreated with CSE. The results showed that CSE effectively inhibited the proliferation of A431. Furthermore, CSE-mediated cell cycle arrest in S phase. We also observed that CSE treatment led to down-regulation of MMP2 and MMP9 and suppress the migration and invasion in A431. CSE exerted its anti-proliferation and anti-migration by targeting EGFR and related metastasis factors, thus could be a useful therapeutic candidate for high-EGFR expression cancer intervention.
 Video Clip of Methodology:
 Cell migration and invasion assay: 2 min 41 sec Full Screen Alternate
Highlights
Now-a-days, insects have proved to be more important as sources of drugs from the Traditional Chinese Medicine
These findings indicated that Centipede Scolopendra extracts (CSE) had potential antitumor properties in A431 in vitro
These data were consistent with results from cell growth inhibition studies above using the MTT, confirming that CSE could act as an inhibitor of A431 cell growth
Summary
Now-a-days, insects have proved to be more important as sources of drugs from the Traditional Chinese Medicine. Centipedes, the oldest terrestrial arthropods and the ecologically important group of soil and leaf litter predators, have been used to treat a variety of conditions, such as spasm, seizure, poisonous nodules, diphtheria (Undheim and King, 2011; Ma et al, 2015), and inhibit the growth of tumor both in vitro and in vivo (Lee et al, 2015). Centipedes have venom glands in their first pair of limbs and their venoms contain a large number of components with different biochemical and pharmacological properties to paralyze and kill preys and to defend against predators. Epidermal growth factor receptor (EGFR) normally promotes cell growth and development, and when mutated, allows the uncontrolled growth of cells that is the hallmark of cancer (Goldman et al, 1996). EGFR increment is an important promoting factor in the infiltration and transition of carcinoma.
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