Abstract
Introduction: Centella asiatica is a herb, which has neuroprotective properties, thus, it can be used to prevent neural damage caused by stress and increased glucocorticoid level. Madecassoside, asiaticoside, asiatic acid, and madecassic acid that are contained inside Centellaasiatica extract have antioxidant and neuroprotective properties.
 Method: This research is an experimental research using an animal model with post test-only control group design. We used samples from biologically stored product, in the form of 25 rat-brain tissue paraffin blocks. These brain tissues originated from 25 rat models that were divided into groups P1, P2, P3, P4, and P5. Group P1 was intervened with restrain stress.Group P2, P3, and P4 was intervened with restrain stress and ethanolic extract of Centella asiaticaat 150, 300, and 600 mg/kgBW/days, respectively. Group P5 was intervened with stress and fluoxetinat 10 mg/kgBW/days. On day 23, brain tissues were terminated, perfused, and extracted. Brain tissues were then made into paraffin blocks, turned into preparation pieces, histochemically stained, and cells that expressed caspace-bax were counted.
 Results: There was a statistically significant difference of Bax expression between P1 group and P3, P4, and P5 (p=0,01). Bax expression in group P1 was significantly higher than P2, P3, P4, and P5. There was no statistically significant difference of Bax expression between group P1 and P2 (p=0,08).
 Conclusion: Ethanolic extract of Centella asiatica was able to decrease Bax expression in prefrontal cortex of chronic restraint stress induced Spraque Dawley rat models.
 Bangladesh Journal of Medical Science Vol.18(1) 2019 p.112-117
Highlights
Centella asiatica is a herb, which has neuroprotective properties, it can be used to prevent neural damage caused by stress and increased glucocorticoid level
In this research, bax expression was found to be higher in stress group (P1) compared to groups which were intervened with Centella asiatica extract
This result indicated that the administration of Centella asiatica ethanolic extract in the dosage of[300] and 600 mg/kgBW/ day for 21 dayscoulddecrease bax expression in the prefrontal cortex, decreasing the amount of cells undergoing apoptosis
Summary
Centella asiatica is an herb that has neuroprotective properties, it can be used to prevent neural damage caused by stress and increased glucocorticoid level. The medial prefrontal cortex is an area of the human brain that is importantfor cognitive integration, emotion, and concentration focus of received information. It has a high number of glucocorticoid receptors, it is influenced by stress. Repeated stress is thought to cause a one third loss of total axons and apical dendrite synapse of pyramidal neurons in the medial prefrontal cortex. This condition may be due to neuroplasticity failure caused by stress exposure[5]. Syaefudin Ali Akhmad, Biochemistry Department, Faculty of Medicine, Universitas Islam Indonesia
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