Abstract
The DNA fragment encoding the cellulose binding domain of endoglucanase III (CBDEG III) from Trichoderma reesei was subcloned and expressed in E. coli. The CBDEG III had a high affinity for cellulose. The morphological and structural changes of cellulose after treatment with CBDEG III indicated a 17% decrease in number of hydrogen bonds and a 16.5% decrease in crystalline index. X-ray diffraction and IR spectra analyses indicated that the destabilization and breakage of the hydrogen bonds in crystalline cellulose accounted for the non-hydrolytic disruption of the structure of cellulose.
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