Abstract
Development of bioseparation media that can perform separations based on both ion exchange and size exclusion provide a transformative technology to purify therapeutic proteins with fewer operations in downstream processing. Thus, in this study, we have developed cellulose-graft-polyethyleneamidoamine (CL-g-PEAA) anion-exchange nanofiber membrane adsorbers with a narrow effective pore size distribution using redox polymerization. Structural characterization illustrated the functionalization of each nanofiber of the CL-g-PEAA membrane adsorbers with three-dimensional nanolayers of amidoamine functionality. The ion-exchange separation efficiency of the CL-g-PEAA anion-exchange membranes, evaluated using bovine serum albumin (BSA), exhibited an excellent static adsorption of 239 mg/g of nanofibers. The BSA dynamic binding capacity in flow through mode at 10% breakthrough of these membranes was 69 mg/g (corresponding to a volume capacity of 31 g/L) with an approximate residence time of 8 s. Meanwhile, CL-g-PE...
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