Abstract
An acid mucopolysaccharide (AMPS) mixture may be separated into its individual constituents by eluting the cetyl pyridinium complexes from a cellulose column with 0.35 M and 0.6 M NaCl, disrupting the complexes with 90% ethyl alcohol and eluting further with 55, 45, 30 and 10% concentrations of ethanol. Depending upon the specific AMPS in the mixture, the procedure can be simplified further. Human arterial AMPS, for example, can be adequately partitioned with only two concentrations of alcohol, 30 and 10%. Acetate paper electrophoresis, infrared spectra, chemical analysis, hydrolysis with hyaluronidase and paper chromatography of products of acid hydrolysis of AMPS are used to identify the separated components and to ascertain the adequacy of separation. Different situations encountered during separation of various mixtures of AMPS are discussed.
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