Abstract
Twenty actinomycete isolates capable of utilizing lignocellulosic biomass were isolated from different samples of agricultural field soil, compost and decaying vegetation. Qualitative screening of all isolates during plate assay method using Congo red showed 17 of them producing a clear zone of hydrolysis around the colonies indicating cellulose hydrolytic potential of the isolates. Quantitative estimation of filter paper (FPase) and carboxymethylcellulase (CMCase) activities exhibited by the positive isolates showed that highest CMCase was produced by NAA2 (0.366 IU/ml), while highest FPase activity of 0.075 FPU/ml was observed in NAA14. All of the isolates showed FPase activities comparable to that shown by C. fimi (0.023 FPU/ml) taken as a standard, confirming the cellulose degradation ability of the isolates.
Highlights
The increasing demand for energy and rising cost of rapidly depleting fossil fuels has necessitated the search for renewable energy sources of energy [1]
The present study focuses on isolation of cellulose degrading actinomycetes from different habitats followed with evaluation of their cellulolytic potential
Bioengineering and Bioscience 4(5): 88-94, 2016 actinomycetes, 1gm of the sample was inoculated in a liquid enrichment medium consisting of minimal salt medium
Summary
The increasing demand for energy and rising cost of rapidly depleting fossil fuels has necessitated the search for renewable energy sources of energy [1]. Lignocellulosic biomass, including industrial, forest, agricultural and domestic wastes, is an attractive alternative to the current fossil fuels based sources of energy [2]. Lignocellulose is primarily composed of cellulose, hemicellulose and lignin [3]. The end product of cellulose hydrolysis, i.e., glucose, a fermentable sugar, is the most desired chemical for the synthesis of biofuel ethanol [5]. The degradation of cellulose is a complex process, which can be carried out both chemically as well as enzymatically [6]. An environmentally benign process, mediated through a complex of enzymes known as cellulases. Cellulase enzyme system comprises three classes of enzymes: (1) 1,4-β-endoglucanase, (2) 1,4-β-exoglucanase, and (3)
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