Abstract
Fed-batch cultures of Trichoderma reesei RUT-C30 attained quasi-steady state conditions, in respect of biomass concentration and enzyme production rate, commensurate with a specific cell maintenance coefficient of 0.029 g cellulose.g biomass.−1h−1 and specific cellulase production rate of between 9.6 and 11.9 IU (filter paper activity).g biomass.−1h−1. A maximum enzyme yield of 57 IU.m1−1 at an overall productivity of 201 IU.L.−1h−1 resulted from a cellulose feed rate of 1.0g.L.−1h−1.
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