Abstract
The treatment of straw with sodium hydroxide in order to upgrade its nutritive value and to increase the utilisation of its energy by ruminants has been widely employed in many parts of the world. The aim of the experiment was to assess the fibrolytic activity of the anaerobic fungi in vitro using either untreated or NaOH treated barley straw.Anaerobic fungi were isolated from the rumen of a sheep fed with a diet of hay and lucerne pellets using medium C based on that described by Davies et al. (1993) and as modified by Rezaeian (1996). The Isolates were inoculated into two series of culture media containing either sodium hydroxide treated (TS) or untreated milled straw (US). Five replicate cultures were prepared and incubated at 39 °C over a period of 12 days. The dry matter loss of the substrates and changes in pH of the medium cultures were measured.
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