Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers.

Hui Liu,Wenchao Li,Jie Hu,Jingjing Wang,Min Wang,Yuejun Kang

doi:10.3390/pharmaceutics10030099

Hui Liu, Wenchao Li + Show 4 more

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https://doi.org/10.3390/pharmaceutics10030099

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Journal: Pharmaceutics	Publication Date: Jul 19, 2018
Citations: 4	License type: CC BY 4.0

Affiliation: Southwest University

Abstract

Understanding of the interaction between cells and nanoparticles (NPs) is critical. Despite numerous attempts to understand the effect of several parameters of NPs on their cellular uptake behaviors, such as size, shape, surface chemistry, etc., limited information is available regarding NP rigidity. Herein, we investigate the effect of rigidity on cellular uptake behaviors of NPs, using generation 5 poly(amidoamine) dendrimer as a model. By harnessing the abundant inner cavity, their rigidity could be effectively regulated by forming size-tunable gold NPs. The NPs thus formed were well characterized and displayed similar hydrodynamic size, surface potential, fluorescence intensity, and distinct rigidity (owing to differences in the size of the Au core). Flow cytometry analysis revealed a positive correlation between NP rigidity and cellular uptake of NPs. Confocal microscopic evaluation revealed that the entrapped gold NPs may affect the intracellular localization of the internalized dendrimers. The present findings can potentially guide the preparation of suitable NPs for biomedical applications.

Highlights

The design and synthesis of desirable nanoparticles (NPs) is an important task for developing efficient carriers in the biomedical field
Jiang et al developed a class of novel polymeric core–lipid shell NPs with tunable rigidity to investigate their cellular uptake behaviors
G5 dendrimers were selected as a model system to investigate the effect of NP rigidity on their cellular uptake behaviors

Summary

Introduction

The design and synthesis of desirable nanoparticles (NPs) is an important task for developing efficient carriers in the biomedical field. The interaction between NPs and cells varies depending on their aforementioned properties [14,15,16,17,18]. Jiang et al developed a class of novel polymeric core–lipid shell NPs with tunable rigidity to investigate their cellular uptake behaviors. Based on both experimental data and molecular dynamics simulations, the cell lines used in their study exhibited preferable intracellular uptake of NPs with higher rigidity [23]

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