Abstract
Egg-oil (Charismon©) is known for its beneficial action in wound healing and other skin irritancies and its antibacterial activity. The physiological basis for these actions has been investigated using cells in culture: HaCaT-cells (immortalized human keratinocytes), human endothelial cells in culture (HUVEC), peripheral blood mononuclear lymphocytes (PBML) and a full thickness human skin model (FTSM). Emphasis was on the influence of egg-oil on cell migration and IL-8 production in HaCaT cells, respiration, mitochondrial membrane potential, reactive oxygen (ROS) production and proliferation in HUVEC and HaCaT cells, cytokine and interleukin production in PBML and UV-light induced damage of FTSM. IL-8 production by HaCaT cells is stimulated by egg-oil whilst in phythemagglutin in-activated PBMLs production of the interleukins IL-2, IL-6, IL-10 and IFN-γ and TFN-α is reduced. ROS-production after H(2)O(2) stimulation first is enhanced but later on reduced. Respiration becomes activated due to partial uncoupling of the mitochondrial respiratory chain and proliferation of HaCaT and HUVEC is reduced. Recovery of human epidermis cells in FTSM after UV-irradiation is strongly supported by egg-oil. These results support the view that egg-oil acts through reduction of inflammatory processes and ROS production. Both these processes are equally important in cellular aging as in healing of chronic wounds.
Highlights
Egg-oil (Charismon©) is a spagyric preparation of eggoil from chicken egg yolk [1] with a broad action spectrum in patients
We treat this problem by investigating the influences of egg-oil on epithelial cell migration, inflammatory responses based on lymphocyte activation and resulting in release of inflammatory cytokines from lymphocytes as well as from epithelial cells because both these cell types interact in a complex network [8]
Each single field of cells was separated into two areas by a Teflon block which prevented adhesion of cells to the area covered by the block. 3 days after cell seeding the Teflon blocks were removed and the coverage of the cell free areas by immigrating cells was measured for 4d under control conditions and with egg-oil suspended in the culture medium by sonication at concentrations of 1 : 1000, 1 : 2000, 1 : 5000 and 1 : 10,000
Summary
Egg-oil (Charismon©) is a spagyric preparation of eggoil from chicken egg yolk [1] with a broad action spectrum in patients. It has been reported to prevent atherosclosis by improving membrane elasticity [2], relief of gum inflammation and bleeding when applied by using Egg-oil-containing tooth paste, Egg-oil containing ointment improves burn wound healing as well as healing of ulcers with low scar formation only [3]. It acts against acne and Herpes zoster, and it exerts antimicrobial (against Staphylococcus and E. coli) and anti-inflammatory actions [1, 2, 4].
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