Abstract
BackgroundThis study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials.MethodsPrimary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay.ResultsCells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. Cells adhered to UltraCal® show protrusion such as filopodia. On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Regarding viability between the materials, we found a higher percentage of viability in cells cultured over discs of Biodentine™ and ProRoot®MTA.ConclusionProRoot®MTA and Biodentine™ exhibit a better cellular response of human apical papilla cells in vitro conditions compared to BioRoot® and calcium hydroxide diluted in sodium chloride.
Highlights
Treatment of immature necrotic permanent teeth due to trauma is a clinical challenge mainly because of the lack of root development and the damage to the periapical area comprising Apical Papilla and Hertwig’s epithelial root sheath (HERS) [1]
Tricalcium silicate cements is a group of hydraulic cements with tri and dicalcium silicates as their chief ingredients, which react with water to form calcium silicate hydrate and Calcium hydroxide Ca(OH)2 (CH), and release calcium and silicon ions, among others [10, 11]
Histological analysis of cellularity of cross and longitudinal sections of the teeth Since one of the goals of treating an immature traumatized tooth is the early prevention of pulp necrosis by repairing the pulp tissue rather than performing invasive endodontic therapy, we studied the different cellular population in the young immature premolars
Summary
Treatment of immature necrotic permanent teeth due to trauma is a clinical challenge mainly because of the lack of root development and the damage to the periapical area comprising Apical Papilla and Hertwig’s epithelial root sheath (HERS) [1]. Tricalcium silicate cements is a group of hydraulic cements with tri and dicalcium silicates as their chief ingredients, which react with water to form calcium silicate hydrate and CH, and release calcium and silicon ions, among others [10, 11]. An advantage of these materials is their ability to form hydroxyapatite, creating a bond between dentin and the tricalcium silicate cement, which is useful in obtaining a tight seal during apexification and REPs. It has been proven that hydraulic cements induce dental pulp stem cell differentiation through the regulation of signal molecules, pathways, receptors and transcription control systems [12]. This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials
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