Abstract
Soluble cellular fractions of surface and submerged cultures of Penicillium cyaneo-fulvum, grown in beef heart infusion (BHI) medium supplemented with glucose, showed well-defined fluctuations in proteolytic activity. Four proteases were shown to occur in the cells, and fluctuations in protease specific activity were considered to be related to the appearance and disappearance of individual enzymes. Synthesis of these enzymes seemed to be regulated by the pH of the external medium.Several proteases also occurred in filtrates of cultures grown in BHI–glucose medium. A comparison of intra- and extra-cellular alkaline proteases (optima, pH 10) showed that, although they were similar in many respects, the enzymes could be distinguished by their action on polyamino acids. Inability to detect the extracellular alkaline protease in cell preparations suggested that this enzyme was released into the medium as rapidly as it was produced.
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