Cellular localization of the class I alcohol dehydrogenase transcript in adult rat tissues

Abstract

The mammalian class I alcohol dehydrogenase is the principal enzyme responsible for ethanol metabolism. While it is regarded primarily as a liver-specific enzyme, class I alcohol dehydrogenase is known to be present in a number of extrahepatic tissues. The purpose of the current study is to define the tissue and cellular distribution of the dehydrogenase transcript in four rat tissues previously shown to contain high levels of mRNA: the liver, the proximal small intestine, the colon and the testis. Localization of the transcript was examined in formalin-fixed, paraffin-embedded rat tissues by in situ hybridization using radioactively labelled antisense rat alcohol dehydrogenase RNA probe. In the liver, the dehydrogenase message is localized primarily to the perivenous hepatocytes. In the proximal small intestine and the colon, the message follows a vertical gradient of distribution along the crypt-villus and the crypt-surface epithelium axes, respectively, with the base of the crypt exhibiting the greatest concentration. In the testis, the message is localized primarily to cells in the interstitium. These findings illustrate a highly compartmentalized nature of distribution of the class I alcohol dehydrogenase transcript in the tissues studied and may help to elucidate the metabolic functions of this enzyme in these tissues.