Cellular internalization mechanism of novel Raman probes designed for plant cells.

Keiko Midorikawa,Takuya Asai,Yutaka Kodama,Yu Miyagi,Simon Sau Yin Law,Kousuke Tsuchiya,Keiji Numata,Yasuyuki Ozeki,Takanori Iino

doi:10.1039/d0cb00128g

Abstract

Diphenylacetylene derivatives containing different polymeric components, poly(l-lysine) (pLys) or tetra(ethylene glycol) (TEG) were designed as novel Raman imaging probes with high Raman sensitivity and low cytotoxicity in living plant cells. The pLys-conjugated probe is internalized via an endocytosis-dependent pathway, whereas TEG-conjugated probe most likely induces direct penetration into the plant cells.

Highlights

Keiko Midorikawa,‡a Kousuke Tsuchiya, ‡*ab Simon Sau Yin Law,a Yu Miyagi,a Takuya Asai,c Takanori Iino, c Yasuyuki Ozeki, c Yutaka Kodama *ad and Keiji Numata *ab
Raman microscopy can detect a vibration attributed to a specific chemical structure within target molecules and can be applied to observe molecular behaviour in living cells without invasion or staining
The alkyne group is the best candidate as a Raman probe for modifying target molecules because it produces a specific peak in a silent region of the Raman spectrum,[6] which enables detection of the probe in molecular crowding situations found in living cells

Summary

Introduction

Keiko Midorikawa,‡a Kousuke Tsuchiya, ‡*ab Simon Sau Yin Law,a Yu Miyagi,a Takuya Asai,c Takanori Iino, c Yasuyuki Ozeki, c Yutaka Kodama *ad and Keiji Numata *ab. ISSN 2633-0679 COMMUNICATION Kousuke Tsuchiya, Yutaka Kodama, Keiji Numata et al Cellular internalization mechanism of novel Raman probes designed for plant cells Diphenylacetylene derivatives containing different polymeric components, poly(L-lysine) (pLys) or tetra(ethylene glycol) (TEG) were designed as novel Raman imaging probes with high Raman sensitivity and low cytotoxicity in living plant cells.

Results

Conclusion