Cellular events within peripodial epithelia that accompany evagination of Manduca wing discs: Conversion of cuboidal epithelia to columnar epithelia

Abstract

In the wing disc of Manduca, a sheet of peripodial epithelium completely covers the apical surface of another epithelium destined to form the wing blade. The cubodial cells of the peripodial epithelium not only are attached to a thick basal lamina but also their lateral and basal surfaces are highly convoluted and stain intensely with ruthenium red (RR). In contrast, the columnar cells of the wing epithelium lack both a basal lamina and RR-positive surfaces. During evagination, the RR-positive material disappears and the extent of lateral cell contact within the peripodial epithelium increases. Concurrently with this lateral “zippering”, the entire peripodial sheet contracts and slides over the wing blade epithelium, thereby exposing the wing to the external surface of the insect. Trypsin treatment of Manduca discs accelerates both evagination and the disappearance of RR-positive material from the surfaces of cells in the peripodial epithelium. Apparently contraction of the peripodial sheet and the increase in its lateral cell contacts is accompanied by the disappearance of acidic glycoproteins from its lateral and basal cell surfaces.