Abstract
An improved method for isolating donor cells from amphibian lens tissue is described. Nuclei from the embryonic and adult frog lens cells were transplanted into prepared, parthogenetically activated, enucleated eggs. The activity and stage of development reached by these experimental eggs decreases as the level of differentiation and age of the donor increases. The area of the cell cycle in which the cell is residing when the donor nucleus is transplanted is of importance. The best results, as far as the developmental potentiality of the nucleus is concerned, are obtained when the donor material's nuclei are at their peak of DNA synthesis. Reasons for this increase and the factors which may be responsible for the behaviour of the aging cell are discussed.
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