Abstract
In L cells infected with reovirus, mengovirus, or Newcastle disease virus, three viruses that inhibit cellular DNA synthesis, segments of DNA synthesized during a 30-min pulse with thymidine- 3H are of the same length as those made in uninfected cells. As measured by the technique of DNA autoradiography, the modal length of newly synthesized pulse-labeled segments of DNA is 10–20 μ both in infected and control cells. L cells treated with puromycin synthesize DNA strands of similar length, in spite of a marked reduction in the amount of DNA made. These results, taken with earlier observations on DNA chain elongation by sedimentation analysis, suggest that all three viruses and puromycin inhibit cellular DNA synthesis by inhibiting multifocal initiation of DNA replication. Minor changes in the rate of DNA chain elongation in virus-infected or puromycin-treated cells cannot be excluded.
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