Cellular distributions of AMPA glutamate receptor subunits in fetal ventral mesencephalon transplants in the dopamine-depleted striatum of a rat

Abstract

To demonstrate the cellular distributions of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunits (GluR1, GluR2/3, and GluR4) in the intrastriatal grafts of a rat model of Parkinson’s disease, immunocytochemistry was performed in 6-hydroxydopamine rats with intrastriatal transplants of fetal ventral mesencephalon (VM). In the fetal VM (at embryonic day 15) in which the tyrosine hydroxylase (TH) immunoreactivity was intensely observed, no GluR subunit immunoreactivity was detected. Within the intrastriatal fetal VM grafts containing TH-positive cells, a large number of cells immunoreactive for GluR1 and GluR2/3 were observed. However, the GluR1- and GluR2/3-positive cells tended to locate homogeneously within the grafts and were composed of various cell sizes and shapes, mainly medium-sized and aspiny cells. Weak GluR4-positive cells were seen in the grafts, although in some cases the staining was too faint to see any immunoreactive cells at all. Double immunostaining revealed that a part of TH-positive cells in the grafts was also immunopositive for GluR1 or GluR2/3. Both dopaminergic neurons and nondopaminergic neurons in the VM transplants appear to be modified functionally by glutamatergic afferents via various glutamate receptors, including GluR1 and GluR2/3 and, to a lesser extent, GluR4.