Cellular and tissue mechanisms of recovery processes in Hordeum distichum L. under irradiation

Abstract

Ionizing and UV-B irradiation of barley seedlings increased the number of chromosome aberrations in vegetative and generative meristems and disturbances in microsporo- and microgametogenesis. Other irradiation-induced changes included enhanced cytomixis and an increase in the number of pathologies in meiosis and tetrads and in the polymorphism and disturbances in pollen grains. Damages induced by different irradiation types differ primarily quantitatively. The dynamics of occurrence of chromosome aberrations in the root meristem reciprocally depended on the irradiation dose. Damages induced by low UV doses were detected throughout the ontogeny of plants. Increased irradiation doses activated cytolytic processes during premeiotic interphase, meiosis, and tetrad formation, as well as after the end of gametogenesis. Exposure to maximum doses of UV radiation and moderate doses of γ-radiation restored the fertility of grain pollens. Microsporocytes, microspores, and pollen grain components undergo degeneration primarily via apoptosis. This type of cell death is apparently autonomous, being induced by the cell population itself. The results of this study confirmed the hypothesis on the positive role of cell competition in the recovery processes and adaptation of plants to mutagens.