Cellular and spatial changes in the anuran superior olive across metamorphosis

Abstract

In many vertebrate species, the superior olive in the auditory brainstem plays an essential role in sound source localization. Little is known, however, about the structural and functional changes in this nucleus during development when alterations in head size and shape as well as in inner ear projections are expected to affect the perception of binaural cues. Using stereological techniques, we investigated the changes in several cellular and spatial features of the bullfrog superior olive across metamorphosis, the time period during which the animal transforms from a totally aquatic larva to a semiterrestrial adult. The total number of cells shows a strongly linear increase from hatchling through late larval stages. The number of neurons decreases during metamorphic climax stages, and recovers to pre-metamorphic climax levels in the early post-metamorphic froglet stage. The number of glial cells increases during the early larval period, and remains relatively stable, with no systematic variation, from late larval to froglet stages. The volume of the superior olive increases rapidly in early larval stages, followed by a much-attenuated rate of growth between late larval and froglet stages. These morphological changes may provide a substrate for the functional restructuring of the bullfrog superior olive, shortly before the switch from aquatic to mostly atmospheric hearing.