Abstract
Gap Junctional intercellular communication (GJIC) mediates the direct transfer of low-molecular weight factors from one cell to neighbouring ones. Since such factors include cAMP, inositol triphosphates and calcium, which are important messengers of signal transduction systems, G is believed to play a pivotal role in tissue homoeostasis. Its impairment is, therefore, likely to c functional defects in multicellular organisms. GJIC can be measured by several methods: (a) metaboli co-operation assay, (b) electrophysiological measurement, and (c) dye-transfer assay. At present, th dye-transfer assay is used in many laboratories since it can be applied easily with cultured cells a extended to in vivo situations with some modifications. In addition, molecular probes (cDNA and antibodies) for various connexin species have become available for studies of the molecular mechanis of GJIC modulation; connexins are structural proteins of gap junctions and cDNAs from at least 13 different connexin genes have been cloned. Many tumour-promoting agents have been shown to inhibit GJIC in cultured cells as well as in vivo. Increasing evidence suggests that connexin genes form of tumour suppressor genes and that various control mechanisms of GJIC are liable to disruption by carcinogenic agents.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
